Expression of the Blood-Clotting Factor-VI11 cDNA Is Repressed by a Transcriptional Silencer Located in Its Coding Region
نویسنده
چکیده
Hemophilia A is caused by a deficiency of actor-Vlll procoagulant (NIII) activity. The current treatment by frequent infusions of plasma-derived Nlll concentrates is very effective but has the risk of transmittance of blood-borne viruses (human immunodeficiency virus IHIVI, hepatitis viruses). Use of recombinant DNA-derived Nlll as well as gene therapy could make hemophilia treatment independent of blood-derived products. So far, the problematic production of the Nlll protein and the low titers of the Nlll retrovirus stocks have prevented preclinical trials of gene therapy for hemophilia A in large-animal models. We have initiated a study of the mechanisms that oppose efficient Nlll synthesis. We have established that Nlll cDNA contains sequences that
منابع مشابه
Expression of the blood-clotting factor-VIII cDNA is repressed by a transcriptional silencer located in its coding region.
Hemophilia A is caused by a deficiency of factor-VIII procoagulant (fVIII) activity. The current treatment by frequent infusions of plasma-derived fVIII concentrates is very effective but has the risk of transmittance of blood-borne viruses (human immunodeficiency virus [HIV], hepatitis viruses). Use of recombinant DNA-derived fVIII as well as gene therapy could make hemophilia treatment indepe...
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